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STROMAGICS

Coralie Sengenes

Repair/Regenerate via the circulation of mesenchymal stromal cells between organs

From their development to the end of their lives, most organisms experience a gradual decline in their regenerative capacity. Schematically, each organ can be divided up into a functional compartment, the parenchyma, and a stromal compartment, the stroma, which supports the parenchymal cells of the organ. In the stroma, there is a population of cells with multiple differentiation capacities, the mesenchymal stromal cells (MSCs). MSCs exhibit a strong regenerative potential and are considered key players in the regeneration of damaged tissue. Adipose tissue is, to date, the richest reservoir of MSCs where they are called adipose stromal cells or ASCs. Unexpectedly, this ASC reservoir can be mobilized in response to stresses of inflammatory and/or lesional origin. Once released, ASCs infiltrate the damaged/inflamed organ and participate in its remodeling and/or regeneration.

How are ASCs recruited? What are the functional characteristics of mobilized ASCs? How does the contribution of ASCs to regeneration evolve according to age-related frailty ? All these questions remain unanswered.

The STROMAGICS project consists in deciphering the relationship between stromal dynamics and age-related functional decline. Our scientific objectives are the following:

  1. To identify the molecular and cellular characteristics of mobilizable ASCs and the mechanisms controlling and enabling this original dynamics
  2. To characterize this dynamic in the context of aging

research areas

AXIS 1

Molecular and cellular mechanisms controlling the dynamics of ASCs in response to distant tissue stress

We are analyzing the heterogeneity of ASCs in terms of motility, differentiation capacity and metabolomic profile.

Single Cell RNA Seq analysis
Single Cell RNA Seq analysis (Merrick et al, 2019)
Motility assay (Boyrie et al, 2018)
Differenciation potential and metabolomic profiling
Differenciation potential and metabolomic profiling

AXIS 2

How do ASCs circulate between organs in response to distant tissue stress?

We are trying to identify the paths used by ASCs to circulate and the molecular and cellular mechanisms they use to navigate between organs. In particular the blood pathway and fascia. We are also exploring the control of these dynamics exerted by the nervous system. 

Microfluidic Device (Ian Papautsky)
Microfluidic Device (Ian Papautsky)
Mechano-phenotyping of ASCs (Belotti at al. 2019)
Mechano-phenotyping of ASCs (Belotti at al. 2019)
Fascia (Dr Guimberteau)
Fascia (Dr Guimberteau)
Control by the Nervous System
Control by the Nervous System

AXIS 3

Functional decline and analysis of ASC dynamics

In this part of the STROMAGICS project, we investigate whether functional decline is associated with altered inter-organ dialogue in response to tissue damage in animal models and human.

team members

Coralie Sengenès

Group Leader - PhD CR INSERM

Mireille André

AI CNRS

Corinne Barreau

PhD - IE UPS-UT3

Cécile Dromard Berthezene

PhD - MCF UPS-UT3
Moucharde

Muriel Boube-Trey

PhD - CR CNRS

Xavier Contreras

PhD - CR Inserm
Mouchard

Laurence Dubois

PhD - CR CNRS
DUCOMM~1

Bernard Ducommun

MCU-PH
Mouchard

Jean-Louis Frendo

PhD - CR CNRS

Amandine Girousse

DVM PhD - CR CNRS

Emma Grandgirard

PhD Student
JOURDAN Géraldine

Géraldine Jourdan

MCU-PH ENVT
Ingénieuse

Margaux Labrosse

PhD Student
MATHIE~1

Maxime Mathieu

PhD Student

Sylvie Monferran

PhD - MCF UPS-UT3 Pharmacie
photo equipe eya

Eya Hamza

PhD - ATER UPS-UT3 Pharmacie
CINTAS Pascal

Pascal Cintras

PH

Publications

Les partenaires de l'équipe

Partenaires SCIENTIFIQUES
soutiens financiers