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GOT-IT
Louis Casteilla
Valérie Planat-Benard
GUIDED SELF-ORGANIZATION OF TISSUE FOR INNOVATIVE THERAPEUTICS
A multidisciplinary approach to study tissue degeneration and regeneration processes in adult mammals and during aging
Set up Advanced therapy medicinal products (ATMPs) for regenerative and rejuvenating strategies
Research
Organs/tissues are ecosystems resulting from a dynamic coordination between the parenchymal compartment (specific element of each organ in relation to its function) and the mesenchymal compartment (transversal element providing the most appropriate support and environment for any organ). This latter plays a fundamental role in tissue architecture which supports optimal organ functions. GOT-IT focuses its research on this architecture, the associated mechanical constraints, and at the cellular level studies mesenchymal stem/stromal cells (MSC) as an orchestrator and key indicator of tissue homeostasis.
This multidisciplinary group proposes an original and holistic approach from the creation of numerical and micro-physiological models to the setting up of clinical trials. To do so, the team relies on state-of-the-art technologies such as mechanobiology (AFM), biophotonics (light sheet microscopy, spectral analysis, …etc), 3D tissue engineering and biomaterials. Moreover, GOT-IT members have set up the unique ISO standards French platform for quality control of MSCs as well as the unique European academic platform labelled for bio-distribution and biosafety studies of ATMPs (LabHPEC) within national infrastructure ECellFrance.
Our team has also played a key role in the development and structuring of the INSPIRE scientific project, which aims to better understand human functional decline and to propose rejuvenative therapies to prevent elderly dependency.
team members
Publications
- Leménager H, Fiévet LMA, Guilloton F, Naji A, Descamps JG, Chaput B, Suganuma N, Pagès JC, Sensebé L, Carrière A, Casteilla L, Deschaseaux F. Cell immaturity and white/beige adipocyte potential of primary human adipose-derived stromal cells are restrained by culture-medium TGFβ1. Stem Cells. 2020 Jun;38(6):782-796. doi: 10.1002/stem.3164. Epub 2020 Feb 26. PMID: 32083764
- Kennel P, Dichamp J, Barreau C, Guissard C, Teyssedre L, Rouquette J, Colombelli J, Lorsignol A, Casteilla L, Plouraboué F. From whole-organ imaging to in-silico blood flow modeling: A new multi-scale network analysis for revisiting tissue functional anatomy. PLoS Comput Biol. 2020 Feb 14;16(2):e1007322. doi: 10.1371/journal.pcbi.1007322. eCollection 2020 Feb. PMID: 32059013 Free PMC article.
- Guerville F, De Souto Barreto P, Ader I, Andrieu S, Casteilla L, Dray C, Fazilleau N, Guyonnet S, Langin D, Liblau R, Parini A, Valet P, Vergnolle N, Rolland Y, Vellas B. Revisiting the Hallmarks of Aging to Identify Markers of Biological Age. J Prev Alzheimers Dis. 2020;7(1):56-64. doi: 10.14283/jpad.2019.50. PMID: 32010927
- Carrière A, Lagarde D, Jeanson Y, Portais JC, Galinier A, Ader I, Casteilla L. The emerging roles of lactate as a redox substrate and signaling molecule in adipose tissues. J Physiol Biochem. 2020 May;76(2):241-250. doi: 10.1007/s13105-019-00723-2. Epub 2020 Jan 2. PMID: 31898016 Review.
- Monsarrat P, Kémoun P, Casteilla L, Planat-Bénard V. Broad-Spectrum Antibacterial Effects of Human Adipose-Derived Stromal Cells. Stem Cells Int. 2019 Oct 29;2019:5389629. doi: 10.1155/2019/5389629. eCollection 2019.
- Morgado Brajones J, Clouvel G, Dovillaire G, Levecq X, Lorenzo C. Highly Sensitive Shack–Hartmann Wavefront Sensor: Application to Non-Transparent Tissue Mimic Imaging with Adaptive Light-Sheet Fluorescence Microscopy. Methods and protocols. 2019, 2 (3), 59.
- Chalard A, Joseph P, Souleille S, Lonetti B, Saffon-Merceron N, Loubinoux I, Vaysse L, Malaquin L , Fitremann J . Wet spinning and radial self-assembly of a carbohydrate low molecular weight gelator into well organized hydrogel filaments. Nanoscale. 2019, 11(32):15043-15056.
- Peurichard D, Ousset M, Paupert J, Aymard B, Lorsignol A, Casteilla L, Degond P. Extra-cellular matrix rigidity may dictate the fate of injury outcome. J. Theor. Biol. 2019, 469:127-136.
- Muller S, Ader I, Creff J, Leménager H, Achard P, Casteilla L, Sensebé L, Carrière A, Deschaseaux F. Human adipose stromal-vascular fraction self-organizes to form vascularized adipose tissue in 3D cultures. Sci. Rep. 2019, 9(1):7250.
- Dichamp J, Barreau C, Guissard C, Carrière A, Martinez Y, Descombes X, Pénicaud L, Rouquette J, Casteilla L, Plouraboué F, Lorsignol A. 3D analysis of the whole subcutaneous adipose tissue reveals a complex spatial network of interconnected lobules with heterogeneous browning ability. Sci. Rep. 2019, 9(1):6684.
- Chalard A, Vaysse L, Joseph P, Malaquin L, Souleille S, Lonetti B, Sol JC, Loubinoux I, Fitremann J. Simple Synthetic Molecular Hydrogels from Self-Assembling Alkylgalactonamides as Scaffold for 3D Neuronal Cell Growth. ACS Appl Mater Interfaces. 2018, 10(20):17004-17017.
- Andilla J, Jorand R, Olarte OE, Dufour A, Cazales M, Montagner YLE, Ceolato R, Riviere N, Olivo-Marin JC, Loza-Alvarez P and Lorenzo C. Imaging tissue-mimic with light sheet microscopy: A comparative guideline. Sci Rep 2017, 7,44939.
- Monsarrat P, Kémoun P, Vergnes Jn, Sensebé L, Casteilla L, Planat-Bénard V. Spatial and temporal structure of the clinical research based on mesenchymal stromal cells: A network analysis. Cytotherapy. 2017, 19(1):47-60.
- Lemaitre M, Monsarrat P, Blasco-Baque V, Loubières P, Burcelin R, Casteilla L, Planat-Bénard V, Kémoun P. Periodontal tissue regeneration using syngeneic adipose-derived stromal cells in a mouse model. Stem Cell Transl. Med. 2017, 6(2):656-665.
- Peurichard D, Delebecque F, Lorsignol A, Barreau C, Rouquette J, Descombes X, Casteilla L, Degond P. Simple mechanical cues could explain adipose tissue morphology. J. Theor. Biol. 2017, 429:61-81.
- Masson A, Escandes P, Frongia C, Clouvel G, Ducommun B, and Lorenzo C. High-resolution in-depth imaging of optically cleared thick samples using an adaptive SPIM. Sci Rep. 2015, 5, 16898.
- Vaysse L, Beduer A, Sol JC, Vieu C, Loubinoux I. Micropatterned bioimplant with guided neuronal cells to promote reconnection and improve functional recovery after primary motor cortex insult. Biomaterials. 2015, 58:46-53.
- Bura-Rivière A, Planat-Benard V, Bourin P, Silvestre JS, Gross F, Grolleau JL, Saint-Lebese B, Peyrafitte JA, Fleury S, Gadelorge M, Taurand M, Dupuis-Coronas S, Leobon B & Casteilla L. A phase I trial: the use of autologous adipose-derived stroma/stem cells to treat patients with non-revascusuclarizable Critical Limb Ischemia (CLI). Cytotherapy, 2014, 16(2):245-257.
- de Barros S, Dehez S, Arnaud E, Barreau C, Cazavet A, Perez G, Galinier A, Casteilla L and Planat-Bénard V. Aging-related decrease of human ASC angiogenic potential is reversed by hypoxia preconditioning through ROS production. Mol. Ther. 2013, 21(2):399-408.
- Jorand R, Le Corre G, Andilla J, Maandhui A, Frongia C, Lobjois V, Ducommun B and Lorenzo C. Deep and clear optical imaging of thick inhomogeneous samples. PLoS One. 2012, 7(4):e35795.
- Béduer A, Vieu C, Arnauduc F, Sol JC, Loubinoux I, Vaysse L. Engineering of adult human neural stem cells differentiation through surface micropatterning. Biomaterials 33 : 504-514 (2012).
- Lorenzo C, Frongia C, Jorand R, Fehrenbach J, Weiss P, Mandhui A, Gay G, Ducommun B and Lobjois V. Live cell division dynamics monitoring in 3D large spheroid tumor models using light sheet microscopy. Cell Division. 2011, 6 :22.
- Planat-Bénard V, Silvestre J.S, Cousin B, André M, Nibbelink M, Tamarat R, Clergue M, Manneville C, Saillan-Barreau C, Duriez M, Tedgui A, Levy B, Pénicaud L, Casteilla L. Plasticity of adipose lineage cells towards endothelial lineage: physiological and therapeutic perspectives. Circ. 2004, 109:656-663
- Method for obtaining human brown/beige adipocytes. Patent N°: WO2017009263. Deschaseaux F, Guilloton F, Muller S, Carrière A, Casteilla L, Sensebé L.
- Multiple sample holder for SPIM. Patent N°: USD797831S (2017), EP002624197 (2015). Lorenzo C, Lobjois V, Ducommun B.
- Utilisation de cellules issues du tissu adipeux pour induire la formation d’un réseau vasculaire fonctionnel. Patent N°: INPI 2 859 381 – DV 63441. Casteilla L, Silvestre JS, Planat-Benard V, Levy B, Penicaud L, Tedgui A, pour le CNRS, France.
- Method for selecting bioactive agents by detecting variation induced in cyclic AMP intracellular concentration of a sensitive living cell. Patent N°: EP1493035 (2003). Furger C, Lorenzo C.
Les partenaires de l'équipe
- P. Degond (Imperial College London, London)
- D. Peurichard (INRIA, Paris)
- C. Maugis-Rabusseau (IMT, Toulouse)
- L. Malaquin, C. Thibault, E. Dague – Equipe Elia (LAAS,Toulouse)
- S. Cussat-Blanc (IRIT, Toulouse)
- F. Plouraboué (IMFT, Toulouse)
- C. Soulé-Dupuy (IRIT, Toulouse)
- Faculty of Odontology of Paul Sabatier University of Toulouse
- Toulouse Institute of Oral Sciences and Medicine
- Toulouse University Hospital Center
- A.-F. Mingotaud – Equipe Ideas (IMRCP, Toulouse)