One of the challenges in biological imaging is to visualize in depth and in three dimensions biological structures and living organisms. CERT provides the tools and expertise to perform this type of analysis. Amongst such tools, the SPIM, the result of research by CERT and other Toulouse laboratories, remains a unique platform instrument to probe biological samples in 3D.
A new microscope based on a light sheet excitation, the SPIM (Selective Plane Illumination Microscope), allows rapid optical sectioning in 3D and in depth on living samples and offers multi-views acquisition opening new perspectives in biological observation. This unique equipment is now available to the entire scientific community.
Specifications
Applications
The MacroSPIM, a macroscope based on a light sheet excitation, makes it possible after optical clearing to visualize tissues and whole organs by rapid in depth 3D optical sectioning. This novel approach offers multi-view acquisition possibilities and opens up new perspectives in biological observation. This unique equipment, based on developments carried out in Barcelona (IRB), is now made available to the entire scientific community by CERT.
Specifications
Applications
Specifications
AxioExaminer (straight stand) LSM 880 (Zeiss)
Maximum distance under the lens: 11 cm
Quasar spectral detection system with 2 lateral PMTs and 1 central GaAsP spectral detector composed of 32 high sensitivity detectors)
Laser 458, 488,514,561 and 633
Ti / Sa Chameleon Laser (Coherent)
Applications
Live imaging (explants, animal models)
Acquisition of up to 10 individual channels (8 GaAsP)
Simultaneous spectral acquisition from 391 to 749 nm
Free setting of the fluorescence emission window.
Confocal acquisition
SHG
Videomicroscopy allows long-term dynamic monitoring of “living” 2D samples.
Specifications
Applications
The Fortessa X20 is equipped with 5 lasers: 488nm blue, 405nm purple, 355nm UV, 640nm red, 561nm yellow-green, which can read 19 parameters. It is equipped with a 96-well plate changer. The platform is also equipped with DIVA, Flowlogic and Kaluza analysis software, which can be used on site under reservation conditions.
The MACSQuant is equipped with 3 lasers: 405nm, 488nm and 640nm, which can read 10 parameters. This cytometer is also equipped with a plate changer, a marking and automatic dilution module. It allows to standardize experiments.
The sorter proposed, an ARIA Fusion (BD Biosciences), is housed under sterile environment and is equipped with 4 lasers: 561nm yellow-green, 407nm violet, 633nm red, 488nm blue, able to read 18 parameters.
This sorter is also equipped with the following accessories:
The platform has an imaging flow cytometer: Image Stream X Mark II (Merck-Luminex). This apparatus is equipped with 3 objectives (20x, 40x and 60x), 3 lasers (405nm, 488nm and 642nm) and a camera. It can read 6 parameters simultaneously.
The image files consisting of tens of thousands of cell images can be routinely analyzed using the IDEAS software. This Software can analyse up to 500 morphometric and photometric parameters for each cell image using patented algorithms. The software is present on the platform and can be used under reservation conditions.
Coupled with a Zeiss inverted fluorescence microscope
Coupled with a Nikon inverted fluorescence microscope
Gesim soft lithography machine (Micro-Contact Printing – µCP)
Soft UV-NIL (Inking, Drying, Molecular deposition, UV curing)
Plasma oxygen microwave DIENER Pico & MEMMERT oven
Microscope droit avec champ sombre
Spin coater (LAURELL WS-650-SZ)
2 MSC (Microbiological safety cabinet) with carbon filter for solvents
2 Incubators and 1 reactor
Specifications | Applications |
| 3 Imaris licences (Bitplane) | 3D & 4D modeling, 2D & 3D tracking |
| 2 Amira licences (ThermoFisher) | 3D modeling, 3D & 4D image processing |
| 1 Vision 4D licence (Arivis) | 3D & 4D modeling, pixel & object classification |
| Matlab licence server (Mathworks) | Pixel & Object classification, workflow & automatic image processing, GUI implementation, database implementation |
| 3 NIS elements licences(Nikon) | 3D & 4D modeling, 2D & 3D tracking, Volume analysis, High content analysis |
| Ilastik open-source software | Pixel & Object classification |
| Fiji open-source software | 2D, 3D & 4D image processing, Pixel & Object classification, 2D & 3D tracking, automatic image processing |
| Python open-source software | Pixel & Object classification, GUI implementation, database implementation |
| Graphpad Prism Licences | Statistical analysis of biological data |
| 3 Flowlogic licences | Flow Cytometry analysis |
| 5 Kaluza licences | Flow Cytometry analysis |
| 1 INSPIRE licence | Imaging Flow Cytometry analysis |
Specifications
Applications
Specifications
Applications
Quantification and purity control based on sample absorbance
UV-visible (190-840nm) measurements
Micro-volumes sampling (1-2µl)
GeneAtlas system microarray – Affymetrix
FX96 Touch Real-Time PCR System- Biorad
StepOnePlus™ Real time PCR – Biorad
Système LightCycler® 480 plateforme PCR – Biorad
Specifications
Gas anesthesia tables (TEM SEGA) for animal catheterization
Device for perfusion on vigil mice (syringe pumps, tether, swivels)
Thermostatic chamber
Applications
Specifications
Applications
